Monoclonal antibodies specific for human tumor-associated antigen 90K/Mac-2 binding protein: Tools to examine protein conformation and function

Paneth cells are morphologically well characterized but their function has been not elucidated. Previously, we identified and purified a 90 KD zinc-binding protein (ZBPP-1) in rat intestine that was localized to Paneth cell granules, consistent with their high zinc content. To further elucidate the structure and function of ZBPP-1, we immunized Balb/c mice with purified ZBPP-1 and identified four independent monoclonal antibodies (MAb) producing MAb ZIP-1 (IgM), ZIP-2 (IgG1), ZIP-3 (IgM), and ZIP-4 (IgM). Immunohistochemistry (IHC) and immunoelectron microscopy (IEM) with these MAb showed positive staining of Paneth cell cytoplasmic granules. MAb ZBPP-1 also stained a population of mononuclear cells in the lamina propria of digestive tract mucosa and a few cells in spleen, presumably a subset of macrophages. These MAb will provide a useful tool to study the function of Paneth cells in human health and disease, since they cross-reacted with human intestinal Paneth cells and mucosal mononuclear cells.

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Faculty Opinions recommendation of The role of interphotoreceptor retinoid-binding protein on the translocation of visual retinoids and function of cone photoreceptors.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1148033.605141 ◽

2009 ◽

Author(s):

Gordon Fain

Keyword(s):

Binding Protein ◽

Cone Photoreceptors ◽

Interphotoreceptor Retinoid Binding Protein ◽

And Function

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Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila

Protein and Peptide Letters ◽

10.2174/0929866526666190327143335 ◽

2019 ◽

Vol 26 (6) ◽

pp. 414-422

Author(s):

Jia Liu ◽

Ping Song ◽

Jie Zhang ◽

Ziyan Nangong ◽

Xiaobei Liu ◽

...

Keyword(s):

Antifungal Activity ◽

Growth Inhibition ◽

Insecticidal Activity ◽

Spore Germination ◽

Binding Protein ◽

Colloidal Chitin ◽

Inhibition Rate ◽

Xenorhabdus Nematophila ◽

Chitin Binding Protein ◽

And Function

Background: Genome sequence analysis (GenBank access No.: FN667742.1) shows that Xenorhabdus nematophila ATCC19061 contains one gene (Xn-cbp) encoding chitin binding protein (Xn-CBP). Objective: The present work aims to clarify the characteristics and function of Xn-CBP from X. nematophila HB310. Methods: In this study, the Xn-cbp gene was cloned and expressed in Escherichia coli BL21 (DE3). Substrate binding assays were performed to explain the ability of Xn-CBP combined with the polysaccharide. The insecticidal toxicity of Xn-CBP against the second-instar larvae of Helicoverpa armigera was determined by feeding method. Besides, the antifungal activity of Xn-CBP against Coniothyrium diplodiella, Verticillium dahlia, and Fusarium oxysporum was tested by spore germination assay and hyphal extension assay. Results: Xn-CBP encoded 199 amino acids with a calculated mass of 28 kDa, which contained a signal peptide and a chitin binding domain. The Bmax and Kd values of Xn-CBP to colloidal chitin were 2.46 and 4.08, respectively. Xn-CBP had insecticidal activity against the H. armigera with a growth inhibition rate of 84.08%. Xn-CBP had the highest spore germination inhibitory effect on C. diplodiella with the inhibition rate of 83.11%. The hyphal growth inhibition rate of Xn-CBP to F. oxysporum, 41.52%, was higher than the other two fungi. Conclusion: The Xn-CBP had the highest binding ability to colloidal chitin and it showed insecticidal activity and antifungal activity. The present study laid a foundation for further exploitation and utilization of X. nematophila.

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Targeting Pin1 for Modulation of Cell Motility and Cancer Therapy

Biomedicines ◽

10.3390/biomedicines9040359 ◽

2021 ◽

Vol 9 (4) ◽

pp. 359

Author(s):

Hsiang-Hao Chuang ◽

Yen-Yi Zhen ◽

Yu-Chen Tsai ◽

Cheng-Hao Chuang ◽

Ming-Shyan Huang ◽

...

Keyword(s):

Cancer Therapy ◽

Tumor Suppressors ◽

Protein Conformation ◽

Genome Stability ◽

Recent Progress ◽

Multiple Roles ◽

Cancer Development ◽

Cancer Hallmarks ◽

Underlying Mechanisms ◽

And Function

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) specifically binds and isomerizes the phosphorylated serine/threonine-proline (pSer/Thr-Pro) motif, which leads to changes in protein conformation and function. Pin1 is widely overexpressed in cancers and plays an important role in tumorigenesis. Mounting evidence has revealed that targeting Pin1 is a potential therapeutic approach for various cancers by inhibiting cell proliferation, reducing metastasis, and maintaining genome stability. In this review, we summarize the underlying mechanisms of Pin1-mediated upregulation of oncogenes and downregulation of tumor suppressors in cancer development. Furthermore, we also discuss the multiple roles of Pin1 in cancer hallmarks and examine Pin1 as a desirable pharmaceutical target for cancer therapy. We also summarize the recent progress of Pin1-targeted small-molecule compounds for anticancer activity.

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Differential effects of monoclonal antibodies on activating transcription factor-1 and cAMP response element binding protein interactions with DNA.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)31629-6 ◽

1994 ◽

Vol 269 (51) ◽

pp. 32254-32263

Author(s):

D.J. Orten ◽

J.M. Strawhecker ◽

S.D. Sanderson ◽

D. Huang ◽

M.B. Prystowsky ◽

...

Keyword(s):

Transcription Factor ◽

Monoclonal Antibodies ◽

Protein Interactions ◽

Binding Protein ◽

Camp Response Element Binding ◽

Camp Response Element ◽

Response Element ◽

Element Binding Protein ◽

Activating Transcription Factor ◽

Transcription Factor 1

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Molecular and Functional Characterization of One Odorant-Binding Protein Gene OBP3 in Bemisia tabaci (Hemiptera: Aleyrodidae)

Journal of Economic Entomology ◽

10.1093/jee/toz248 ◽

2019 ◽

Cited By ~ 1

Author(s):

Ran Wang ◽

Yuan Hu ◽

Peiling Wei ◽

Cheng Qu ◽

Chen Luo

Keyword(s):

Host Plant ◽

Bemisia Tabaci ◽

Binding Protein ◽

Insect Pest ◽

Critical Role ◽

Functional Characterization ◽

Odorant Binding Protein ◽

Binding Characteristics ◽

And Function ◽

Odorant Binding

Abstract Odorant binding proteins (OBPs) of insects play a critical role in chemical perceptions and choice of insect host plant. Bemisia tabaci is a notorious insect pest which can damage more than 600 plant species. In order to explore functions of OBPs in B. tabaci, here we investigated binding characteristics and function of odorant-binding protein 3 in B. tabaci (BtabOBP3). The results indicated that BtabOBP3 shows highly similar sequence with OBPs of other insects, including the typical signature motif of six cysteines. The recombinant BtabOBP3 protein was obtained, and the evaluation of binding affinities to tested volatiles of host plant was conducted, then the results indicated that β-ionone had significantly higher binding to BtabOBP3 among other tested plant volatiles. Furthermore, silencing of BtabOBP3 significantly altered choice behavior of B. tabaci to β-ionone. In conclusion, it has been demonstrated that BtabOBP3 exerts function as one carrier of β-ionone and the results could be contributed to reveal the mechanisms of choosing host plant in B. tabaci.

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